Chiral separation media

ABSTRACT

The media is an ether derivative of cyclodextrin such as a permethylated hydroxy ether of cyclodextrin or a dialkyl trifluoroacetyl ether derivative of cyclodextrin and is used in capillary gas chromatographic columns. Particular benefit is obtained using the ether derivative of cyclodextrin in a fused silica capillary.

This application is a continuation-in-part of U.S. Pat. application Ser. No. 406,141 filed Sept. 12, 1989 and now U.S. Pat. No. 4,948,395.

This invention relates to a composition used for separation of optical isomers or chiral compounds, as well as other isomeric and non-isomeric compounds, by means of gas chromatography and, more particularly, to the separation of enantiomers by capillary gas chromatography. The composition used is a media of an ether derivative of a cyclodextrin such as a permethylated hydroxy or a dialkyl trifluoroacetyl ether of cyclodextrin.

The configuration of a chiral molecule is generally what determines its biological and pharmaceutical activity and effectiveness. One of the enantiomers of the chiral molecule may be active and the other may be inactive or even toxic. It is therefore essential to be able to separate the different enantiomers from each other to obtain an isolated enantiomer in pure form. The isolated enantiomers are used in the pharmaceutical, pesticide and/or herbicide industries, among others.

A conventional way to separate enantiomers is by gas chromatography using a chiral stationary phase. Chiral stationary phases can rapidly and reversibly form diastereomeric association complexes with chiral analytes. Successful chiral stationary phases for wall-coated capillary tubes should have some characteristic properties. They need to be highly viscous even at elevated temperatures and have the proper surface tension to wet the capillary wall completely. They should be able to form rapid and reversible diastereomeric associate complexes with the chiral analytes via various interactions such as hydrogen bonding, dispersion, dipole-dipole and steric interactions to give reasonable chiral selectivity. Other desirable properties include high temperature stability, no racemization at elevated temperatures and low levels of bleeding.

Most of the early work on chiral stationary phases for gas chromatography used amino acids, peptides, and various derivatives thereof. Some efforts have been made to use other naturally occurring chiral molecules as chiral stationary phases such as tartaric acid, malic acid, mandelic acid and chrysanthemic acid. Despite a large amount of work in this area, the only resulting widely available and commercially viable chiral stationary phase for gas chromatography has been Chirasil-Val from Macherey-Nagel of the Federal Republic of Germany which consists of a siloxane copolymer to which L-valine-tert-butylamide was coupled.

There are a number of limitations to these early amino acid based gas chromatography chiral stationary phases. First, they do not seem to be widely applicable. Most of the reported separations were of racemic amino acid derivatives. Just as significant was the fact that, using these early chiral separation phases, the high column temperature needed for gas chromatography often results in racemization, decomposition and bleeding of the chiral stationary phase. Even the moderately successful Chirasil-Val is not recommended to be used at temperatures much above 200° C. Also, the enantioselectivity of the chiral stationary phase decreases significantly at the higher temperatures needed for gas chromatography.

Previously there have been a number of efforts to use alpha- and beta-cyclodextrin as gas chromatography stationary phases. It was apparent from the early gas chromatography and more recent liquid chromatography work that cyclodextrins had potential as gas chromatographic stationary phases. Unfortunately, the cyclodextrin gas chromatography stationary phases were not as successful as the liquid chromatography bonded stationary phases. Although interesting selectivities, mainly for achiral solutes, were obtained, the efficiency and reproducibility were less than desirable. Cyclodextrins are crystalline solids and had to be dissolved or suspended in another solvent prior to coating the separation column. The fact that native cyclodextrins and their simple derivatives (dimethyl, acetyl, etc.) are crystalline solids with high melting or decomposition points, makes them difficult to use directly as gas chromatography stationary phase coatings. Recently, there have been reports on derivatized cyclodextrins that are liquids or lower melting point amorphous solids and therefore can be used directly as stationary phase coatings. All of these previous compounds are lipophilic derivatives of cyclodextrin (such as perpentyl-beta-cyclodextrin).

Cyclodextrins (also called "Schardinger dextrins") are known to be cyclic oligosaccharides composed of glucose residues bonded together by alpha 1,4 bonds. The six, seven and eight membered rings are called alpha-, beta-, and gamma-cyclodextrin, respectively. The cyclodextrins have different chemical and physical properties from the linear oligosaccharides derived from starch in that they are non-reducing dextrins and the ring structure is widely used as a host for the inclusion of various compounds, usually organic compounds for the food, pharmaceutical, and chemical fields.

As is also well-known, cyclodextrins are produced from starch of any selected plant variety such as corn, potato, waxy maize and the like which may be modified, or unmodified starch derived from cereal or tuber origin and the amylose or amylopectin fractions thereof. The selected starch in aqueous slurry at selected concentrations up to about 35% by weight solids is usually liquefied as by gelatinization or treatment with a liquefying enzyme such as bacterial alpha-amylase enzyme and then subjected to treatment with a transglycosylase (CGT) enzyme to form the cyclodextrins.

The amount of the individual alpha-, beta- and gamma-cyclodextrins produced by treating the starch with the CGT enzyme will vary depending on the selected starch, selected CGT enzyme and processing conditions. The parameters to select for the CGT enzyme conversion for the desired result in the amount of each individual cyclodextrin to be produced is conventional and well-described in the literature.

Conventionally, the DE of the liquefied starch is maintained below about 20 DE, the starch solids concentration is below about 35% by weight, the pH for conversion may be about 4.5 to 8.5 at a selected temperature from ambient up to about 75° C. for a selected period of time, typically from about 10 hours up to seven days and more. The amount of CGT enzyme used for conversion is conventional and well-known in the art.

It has now been discovered that ether derivatives of cyclodextrin such as permethylated hydroxy ethers of cyclodextrin and dialkyl trifluoroacetyl ethers of cyclodextrin can be used to separate a wide variety of optical isomers. The optical isomers resolved include chiral alcohols, diols, polyols, amines, amino alcohols, halohydrocarbons, ketones, lactones, alpha-halocarboxylic acid esters, carbohydrates, epoxides, glycidyl analogues, haloephihydrins, nicotine compounds, pyrans, furans, bicyclic and heterocyclic compounds and other miscellaneous compounds.

The permethylated hydroxy ether of cyclodextrin is hydrophilic and relatively polar compared to previous cyclodextrin derivatives used as chiral stationary phases. The dialkyl trifluoroacetyl derivative is of intermediate polarity. Both of these ether derivatives of cyclodextrin have better coating properties on fused silica capillaries than the hydrophobic cyclodextrin derivatives. The more hydrophilic permethylated hydroxy ether of cyclodextrin and the dialkyl trifluoroacetyl derivatives have different selectivities for the enantiomers as compared to the hydrophobic cyclodextrin derivatives. Also, they can be used at higher temperatures than Chirasil-Val without racemization.

With respect to the ether derivative of cyclodextrin which is a permethylated hydroxy cyclodextrin, the degree of substitution (DS) of the hydroxy ether groups for the hydroxyl groups on the underivatized cyclodextrin molecule is from about 10% to about 75% of the available hydroxyl groups on the cyclodextrin. For example, for alpha-cyclodextrin the DS for the hydroxy ether groups is about 2 to about 12; for beta-cyclodextrin, about 2 to about 14; and for gamma-cyclodextrin, about 2 to about 16. More preferably, the DS is about 25% to about 60% of the available sites and more preferred is about 40%. It is important that the degree of substitution of the cyclodextrin hydroxy groups by the ether side chains be random and non-uniform.

The DS of the methyl groups for the remaining hydroxyl groups on the cyclodextrin and the hydroxyl groups on the ether side chains of the cyclodextrin is greater than about 90% and more preferably above about 95%. Ideally, all of the available hydroxyl sites on the hydroxy ether of cyclodextrin are methylated; although this is sometimes difficult to achieve.

The permethylated hydroxy ether of cyclodextrin is made in a conventional manner starting from either individual cyclodextrin or mixtures of the alpha-, beta- and/or gamma-cyclodextrins. However, it is preferred to use only a single cyclodextrin, i.e. either alpha-, beta-, or gamma-cyclodextrin. The separation and/or purification of the alpha-, beta-, and gamma-cyclodextrin may be done before, after, or at any stage of the derivatization process. The procedure for making the hydroxy ether of cyclodextrin and the subsequent step of methylating are accomplished in a conventional manner. The order of reaction for forming the permethylated hydroxy ether cyclodextrin derivative is first to make the hydroxy ether and then to methylate it.

In order to etherify the underivatized cyclodextrin, the cyclodextrin is suitably reacted with an epoxide. Suitable epoxides include ethylene oxide, propylene oxide, butylene oxide, amylene oxide, glycidol (hydroxypropylene oxide), butadiene oxide, glycidyl methyl ether, glycidyl isopropyl ether, alkyl glycidyl ether, styrene oxide, and t-butyl glycidyl ether. Preferably, the epoxide is selected from the group consisting of propylene oxide, glycidyl methyl ether, glycidyl isopropyl ether, alkyl glycidyl ether, t-butyl glycidyl ether and styrene oxide. Good results have been obtained with an epoxide selected from the group consisting of propylene oxide, glycidyl methyl ether and glycidyl isopropyl ether.

As is known, the reaction of cyclodextrin with the above noted epoxides produces an ether of cyclodextrin with two hydroxy groups on adjacent carbon atoms on the side chains. Such hydroxy ethers of cyclodextrin are sometimes referred to as dihydroxyalkyl ethers of cyclodextrin.

The preferred permethylated ether derivatives of cyclodextrin for use in the present invention include hydroxy propylated cyclodextrin, hydroxy propylated methyl ether cyclodextrin, hydroxy propylated isopropyl ether cyclodextrin, hydroxy propylated vinyl ether cyclodextrin, hydroxy propylated t-butyl ether cyclodextrin, and hydroxy ethylated phenyl cyclodextrin. Good results have been obtained with hydroxy propylated cyclodextrin, hydroxy propylated methyl ether cyclodextrin and hydroxy propylated isopropyl ether cyclodextrin.

With respect to the ether derivative of cyclodextrin which is a dialkyl trifluoroacetyl cyclodextrin, the degree of substitution (DS) of the alkyl groups for the hydrogens of the hydroxyl groups on the underivatized cyclodextrin molecule to form the etherified derivative is from about 20% to about 80% of the available hydrogens on the cyclodextrin. For example, for alpha-cyclodextrin the DS for the alkyl groups is about 1 to about 15; for beta-cyclodextrin, about 1 to about 16; and for gamma-cyclodextrin, about 1 to about 18. More preferably, the DS is about 40% to about 70% of the available sites and more preferred is about 65%. Generally, the alkyl groups will substitute for the hydrogens on the 2 and 6 carbons. It is important that the degree of substitution of the cyclodextrin hydroxy groups by the ether side chains be random and non-uniform.

The DS of the trifluoroacetyl group for the hydrogen of the remaining available hydroxyl group on the cyclodextrin is greater than about 90% and more preferably above about 95%.

The dialkyl trifluoroacetyl ether of cyclodextrin is made in a conventional manner starting from either individual cyclodextrin or mixtures of the alpha-, beta- and/or gamma-cyclodextrins. However, it is preferred to use only a single cyclodextrin, i.e. either alpha-, beta-, or gamma-cyclodextrin. The separation and/or purification of the alpha-, beta-, and gamma-cyclodextrin may be done before, after, or at any stage of the derivatization process. The procedure for making the ether of cyclodextrin and the subsequent step of esterifying are accomplished in a conventional manner. The order of reaction for forming the cyclodextrin derivative is first to make the ether derivative and then to esterify it.

In order to form the dialkylated ether derivative of cyclodextrin, the cyclodextrin is first suitably reacted with an alkyl halide to form an ether derivative. Suitable alkyl halides include chlorinated, brominated, iodiated or fluorinated alkanes such as methane, ethane, propane, butane, pentane or hexane. The alkanes may be straight-chained or branched. Preferably, the alkyl halide is selected from the group consisting of n-propyl bromide, n-propyl chloride, n-butyl chloride, n-butyl bromide, n-pentyl bromide and n-pentyl chloride. Best results have been obtained with n-pentyl bromide.

Suitably, in order to esterify the ether derivative of cyclodextrin, fluorinated anhydrides are used. It is more preferred to use fluorinated anhydrides such as trifluoroacetic anhydride, trifluorobutanoic anhydride, or trifluoropropanoic anhydride. It is most preferred to use trifluoroacetic anhydride.

The preferred dialkyl trifluoroacetic ether derivatives of cyclodextrin for use in the present invention include dipentyl trifluoroacetic ether cyclodextrin, dipentyl trifluoroacetic ether cyclodextrin, dipentyl trifluorobutanoic ether cyclodextrin and dipentyl trifluoropropanoic ether cyclodextrin.

The gas chromatography is performed in a conventional manner.

It is preferred to use fused silica capillaries when performing the gas chromatography operation. Fused silica capillaries are much more flexible, practical and easier to handle than conventional glass capillaries.

The ether derivatives of cyclodextrin of the present invention are liquids at room temperature and can be used to coat undeactivated fused silica capillaries. They are nonvolatile and are thermally stable at temperatures up to about 300° C. in the absence of oxygen.

The gas chromatography can also be performed by administering the ether derivative of cyclodextrin of the present invention to a packed column by means of a carrier such as polysiloxane or polyethylene glycol. The admixture of the ether derivative of cyclodextrin of the present invention with the carrier and the administration of this admixture to the column is performed in a conventional manner.

The solid support in the packed column may be any suitable support such as diatomaceous earth, Teflon powder, or fine glass beads. The selection of the type, size and shape of the solid support depends on the molecules being isolated and the flow rate desired. The determination of which solid support to use is conventional and well-known to those of skill in the art.

Although it is less common, enantioselective reversals also can occur among like-derivatized alpha-, beta-, and gamma-cyclodextrins. Reversals in elution order have been observed between alpha- and beta-cyclodextrin and between beta- and gamma-cyclodextrin.

Further details and advantages of the present invention may be more fully understood by reference to the following examples

EXAMPLE 1

This example illustrates making a permethylated hydroxypropylated ether derivative of cyclodextrin of the present invention.

The permethyl hydroxypropyl ether derivative of cyclodextrin were made in two steps. First, propylene oxide, sodium hydride and the desired cyclodextrin (either alpha-, beta-, or gamma-) were dissolved in dimethyl sulfoxide (DMSO) at 60° C. and allowed to react for one hour with stirring. After cooling to room temperature for 15 hours, the mixture was further cooled in an ice bath. Excess methyl iodide was added dropwise. After 24 hours the reaction was complete

EXAMPLE 2

This example illustrates the making of the dialkyl trifluoroacetyl ether derivative of cyclodextrin of the present invention The alkane used herein is pentane.

3.0 g of dried cyclodextrin and excess 1-bromopentane were added to 30 ml dimethyl sulfoxide (DMSO). The reaction was carried out at 60° C. for 6 hours. Water was then added to the reaction mixture and a waxy precipitate was obtained. The raw product was dissolved in chloroform (CHCl₃) and the solution was washed with water. CHCl₃ was removed under vacuum and the product was used for the next reaction without further purification.

The above material and an excess of trifluoroacetic anhydride were added to 30 ml tetrahydrofuran (THF). The mixture was boiled for 2 hours, then poured over ice to precipitate the product. The precipitate was washed with cold water and dissolved in CHCl₃. The CHCl₃ solution was extracted three times with 5% aqueous sodium bicarbonate (NaHCO₃) and three times with water. The CHCl₃ layer was collected and dried with anhydrous sodium sulfate (Na₂ SO₄). CHCl₃ was allowed to evaporate in a vacuum desiccator and the final viscous liquid was dried in a vacuum overnight to form the final product of dipentyl trifluoroacetyl ether cyclodextrin derivative.

EXAMPLE 3

This example illustrates separating optical isomers using the media made in Example 1 above. The optical isomers were derivatized in order to decrease their volatility for better resolution in the gas chromatograph process.

Fused silica capillary tubes (0.25 mm ID) were obtained from Alltech or Supelco. The capillaries were coated with the cyclodextrin derivatives of the present invention via the static method.

Untreated 10 m capillary columns were placed in a water bath at 36° C. A 0.2% w/v ether solution of the cyclodextrin derivative of the present invention filled the capillary. One end of the capillary was sealed and the other connected to a vacuum line. It took about 4 hours to coat a 10 m column. The column efficiency was tested at 100° C. by using n-hydrocarbons (C11 and C12) as test solutes. Only columns that produced ≧3600 plates per column meter were used.

Racemic mixtures of amines and alcohols to be resolved were derivatized with trifluoroacetic anhydride, acetic anhydride or chloroacetic anhydride. Other compounds containing hydroxyl and/or amine functionalities were also derivitized. All chemicals were obtained from Aldrich Chemical Co., Sigma Chemical Co., or Fluka Chemical Co. In each case, approximately 1.0 mg of the racemic analyte was dissolved in 0.5 ml of methylene chloride and 200 μl of the desired anhydride added. After reacting for about 5-30 minutes, dry nitrogen was bubbled through the solution to remove excess reagent. The solution residue was dissolved in 0.5 ml of ether or methanol for chromatographic analysis. Racemic mixtures of sugars to be resolved were trifluoroacetylated by the above procedure except that tetrahydrofuran was used as the solvent. Also, because this reaction was somewhat slower and the trifluoroacetic anhydride was volatile, three additional aliquots of trifluoroacetic anhydride were added at seven minute intervals.

Both Hewlett Packard (5710A) and Varian (3700) gas chromatographs were used for all separations. Split injection and flame ionization detection were utilized. The injection and detector port temperatures were 200° C. and nitrogen was used as the carrier gas. Gas velocity was about 10-15 cm/sec. A split ratio of 100/1 was used for all the columns and at all of the column temperatures. The injection volume was 0.5 μl. Referring to the following tables, the separation factor, α, is a measure of the separation between the eluted peaks. The greater the separation between the peaks, the greater is α. Mathematically, α is defined as the ratio of the corrected retention times of the two peaks being compared (i.e., ##EQU1## where t'=corrected retention time, t=uncorrected time and t₀ =retention time of an unretained compound. Traditionally, the longest retained peak time, t₂, is put in the numerator so that α will be greater than 1. An α-value of 1 means that there is no separation (the peaks co-elute).

Tables IA, IB and IC illustrate the separation results using permethylated hydroxypropyl ether cyclodextrin (PHCD) as the ether derivative of cyclodextrin. Tables IA, IB and IC show the results using alpha-, beta-, and gamma-cyclodextrin, respectively. The permethylated hydroxypropyl ether cyclodextrin was made in accordance with Example 1 above.

                                      TABLE Ia                                     __________________________________________________________________________     Alpha-Cyclodextrin                                                             (DS by hydroxypropyl = average about 7,                                        ranged between 2-12)                                                                                    Column               Elution                                                   Length                                                                              Temperature     Order                            Structure      Compound  (m)  (°C.)                                                                          R     α                                                                           (if known)                       __________________________________________________________________________     Sugars*                                                                         ##STR1##      allose    9    100                                                                                    ##STR2##                                                                            1.10                                                                              --                                ##STR3##      ribose    9    100                                                                                    ##STR4##                                                                            1.02                                                                              --                               Alcohols                                                                        ##STR5##      isopinocamphenol                                                                         9     90                                                                                    ##STR6##                                                                            1.01                                                                              --                                ##STR7##      trans-1,2-dithiane- 4,5-diol                                                             9    100                                                                                    ##STR8##                                                                            1.12                                                                              --                                ##STR9##      1,2,3,4-tetrahydro- 1-naphthol                                                           9    100                                                                                    ##STR10##                                                                           1.03                                                                              S, R                             Amines                                                                          ##STR11##     3-aminoheptane                                                                           9     90                                                                                    ##STR12##                                                                           1.04                                                                              --                                ##STR13##     2-aminoheptane                                                                           9     90                                                                                    ##STR14##                                                                           1.05                                                                              --                                ##STR15##     1,5-dimethylhexyl- amine                                                                 9     90                                                                                    ##STR16##                                                                           1.04                                                                              --                                ##STR17##     1-phenyl ethyl amine                                                                     9     90                                                                                    ##STR18##                                                                           1.02                                                                              --                                ##STR19##     1,2,3,4-tetrahydro-1- naphthylamine                                                      9    130                                                                                    ##STR20##                                                                           1.03                                                                              --                                ##STR21##     1-(1-naphthyl)- ethylamine                                                               9    150                                                                                    ##STR22##                                                                           1.03                                                                              S, R                             Amino Alcohols                                                                  ##STR23##     2-amino-1-methoxy- propane                                                               9    100                                                                                    ##STR24##                                                                           1.03                                                                              --                                ##STR25##     2-amino-1-propanol                                                                       9    120                                                                                    ##STR26##                                                                           1.02                                                                              --                                ##STR27##     1-amino-2-propanol                                                                       9    120                                                                                    ##STR28##                                                                           1.01                                                                              --                                ##STR29##     2-amino-1-butanol                                                                        9    120                                                                                    ##STR30##                                                                           1.02                                                                              --                               Bicyclic Compounds                                                              ##STR31##     exo-2-amino- norbornane                                                                  9    100                                                                                    ##STR32##                                                                           1.01                                                                              --                                ##STR33##     endo-2-amino- norbornane                                                                 9     90                                                                                    ##STR34##                                                                           1.02                                                                              --                               __________________________________________________________________________      *only Dform is shown                                                     

                                      TABLE Ib                                     __________________________________________________________________________     Beta-Cyclodextrin                                                              (DS by hydroxypropyl = average 8, range 2-14)                                                           Column               Elution                                                   Length                                                                              Temperature     Order                            Structure     Compound   (m)  (°C.)                                                                          R     α                                                                           (if known)                       __________________________________________________________________________     Alcohols                                                                        ##STR35##    trans-1,2-dithiane- 4,5-diol                                                              10   100                                                                                    ##STR36##                                                                           1.06                                                                              --                                ##STR37##    isopinocamphenol                                                                          10    90                                                                                    ##STR38##                                                                           1.10                                                                              --                                ##STR39##    1,2,3,4-tetrahydro- 1-naphthol                                                            20   120                                                                                    ##STR40##                                                                           1.07                                                                              R,S                               ##STR41##    1-indanol  20   110                                                                                    ##STR42##                                                                           1.05                                                                              --                                             trans-cyclohexane- 1,2 diol                                                               30                                                                                          ##STR43##                                                                           1.05                                                                              --                               Amino Alcohols                                                                  ##STR44##    2-amino-1-methoxy propane                                                                 20   110                                                                                    ##STR45##                                                                           1.06                                                                              --                                ##STR46##    2-amino-1-propanol                                                                        20   120                                                                                    ##STR47##                                                                           1.04                                                                              --                                ##STR48##    1-amino-2-propanol                                                                        20   120                                                                                    ##STR49##                                                                           1.06                                                                              --                                ##STR50##    2-amino-1-propanol                                                                        30   120                                                                                    ##STR51##                                                                           1.04                                                                              --                                ##STR52##    2-amino-1-methoxy propane                                                                 30   120                                                                                    ##STR53##                                                                           1.07                                                                              --                                ##STR54##    2-amino-1-butanol                                                                         20   120                                                                                    ##STR55##                                                                           1.05                                                                              --                                ##STR56##    2-amino-3-methyl-  butanol                                                                10   120                                                                                    ##STR57##                                                                           1.03                                                                              --                               Amines                                                                          ##STR58##    2-aminopentane                                                                            9     70                                                                                    ##STR59##                                                                           1.05                                                                              --                                ##STR60##    1,3-dimethylbutyl- amine                                                                  9     70                                                                                    ##STR61##                                                                           1.04                                                                              --                                ##STR62##    3-aminoheptane                                                                            9     80                                                                                    ##STR63##                                                                           1.08                                                                              --                                ##STR64##    2-aminoheptane                                                                            9     80                                                                                    ##STR65##                                                                           1.12                                                                              --                                ##STR66##    1,5-dimethylhexyl amine                                                                   9     80                                                                                    ##STR67##                                                                           1.15                                                                              --                                ##STR68##    1-cyclohexyl- ethylamine                                                                  9    100                                                                                    ##STR69##                                                                           1.05                                                                              --                                ##STR70##    1-phenyl-ethyl-amine                                                                      9    100                                                                                    ##STR71##                                                                           1.04                                                                              --                                ##STR72##    1-aminoindan                                                                              20   150                                                                                    ##STR73##                                                                           1.01                                                                              --                                ##STR74##    1-cyclohexylethyl- amine                                                                  30                                                                                          ##STR75##                                                                           1.05                                                                              --                                             1-(alpha-naphythyl)- ethylamine                                                           30                                                                                          ##STR76##                                                                           1.02                                                                              --                                ##STR77##    1,2,3,4-tetrahydro- 1-naphthylamine                                                       20   150                                                                                    ##STR78##                                                                           1.04                                                                              --                               Nicotine Compounds                                                              ##STR79##               20   140-180                                                                                ##STR80##                                                                           1.01 1.00                                                                         --                               Lactones                                                                        ##STR81##    β,β-dimethyl-γ-  (hydroxymethyl)- γ-bu                   tyrolactone                                                                               9    100                                                                                    ##STR82##                                                                           1.02                                                                              --                                ##STR83##    α-amino-γ-butyrl lactone                                                      30   100                                                                                    ##STR84##                                                                           1.03                                                                              --                               Sugars                                                                          ##STR85##    mannose    9     90                                                                                    ##STR86##                                                                           1.02                                                                              --                                ##STR87##    galactose  9    100                                                                                    ##STR88##                                                                           1.05                                                                              --                                ##STR89##    ribose     9    100                                                                                    ##STR90##                                                                           1.08                                                                              D,L                               ##STR91##    arabinose  9     90                                                                                    ##STR92##                                                                           1.04                                                                              D,L                               ##STR93##    xylose     9     80                                                                                    ##STR94##                                                                           1.03                                                                              L,D                               ##STR95##    lyxose     9     80                                                                                    ##STR96##                                                                           1.04                                                                              D,L                               ##STR97##    erythrose  20   80                                                                                     ##STR98##                                                                           1.03                                                                              L,D                               ##STR99##    sorbose    9    90                                                                                     ##STR100##                                                                          1.12                                                                              L,D                               ##STR101##   1-O-methyl-β-D,L- arabinopyranoside                                                  20    90                                                                                    ##STR102##                                                                          1.09                                                                              D,L                               ##STR103##   glyceraldehyde                                                                            30   130                                                                                    ##STR104##                                                                          1.04                                                                              --                               Bicyclic Compounds                                                              ##STR105##   5-norbornen-2-ol                                                                          9     60                                                                                    ##STR106##                                                                          1.00 1.10                                                                         --                                ##STR107##   exo-norbornenol                                                                           9     70                                                                                    ##STR108##                                                                          1.04                                                                              --                               Miscellaneous                                                                   ##STR109##   butyl lactate                                                                             9     60                                                                                    ##STR110##                                                                          1.04                                                                              --                               __________________________________________________________________________

                                      TABLE Ic                                     __________________________________________________________________________     Gamma-Cyclodextrin                                                             (DS by hydroxypropyl = average about 9,                                        range between 2-16)                                                                                     Column               Elution                                                   Length                                                                              Temperature     Order                            Structure      Compound  (m)  (°C.)                                                                          R     α                                                                           (if known)                       __________________________________________________________________________      ##STR111##    2-amino-1-propanol                                                                       9    120                                                                                    ##STR112##                                                                          1.02                                                                              --                                ##STR113##    1-phenyl-ethylamine                                                                      9    100                                                                                    ##STR114##                                                                          1.04                                                                              --                                ##STR115##    2-aminoheptane                                                                           9     90                                                                                    ##STR116##                                                                          1.02                                                                              --                                ##STR117##    1,5-dimethylhexyl- amine                                                                 9     90                                                                                    ##STR118##                                                                          1.03                                                                              --                                ##STR119##    trans-1,2-dithiane 4,5-diol                                                              9    100                                                                                    ##STR120##                                                                          1.03                                                                              --                                ##STR121##    solketal  9     70                                                                                    ##STR122##                                                                          1.02                                                                              --                               __________________________________________________________________________

EXAMPLE 4

This example illustrates separating non-derivatized optical isomers using the media of permethylated hydroxypropyl ether derivative of cyclodextrin as made in Example 1 above.

The capillary tubes, the gas chromatographs and the determination of α were as set forth in Example 3 above.

Tables IIa, IIb and IIc illustrate the results using alpha-, beta-, and gamma-cyclodextrin, respectively.

                                      TABLE IIa                                    __________________________________________________________________________     Alpha-Cyclodextrin                                                             (DS by hydroxypropyl = average about 7, range between 2-12)                                                Column         Elution                                                         Length                                                                              Temperature                                                                              Order                               Structure        Compound   (m)  (°C.)                                                                          α                                                                           (if known)                          __________________________________________________________________________      ##STR123##      limonene oxide                                                                            9    90     1.06 1.20                                                                         --                                   ##STR124##      limonene   9    70     1.03                                                                              --                                   ##STR125##      carvon     9    100    1.02                                                                              --                                   ##STR126##      4-phenyl-1,3-dioxane                                                                      9    100    1.03                                                                              --                                   ##STR127##      mandelic acid methyl ester                                                                9    120    1.04                                                                              --                                   ##STR128##      mandelic acid ethyl ester                                                                 9    120    1.03                                                                              R, S                                Lactones                                                                        ##STR129##      γ-phenyl-γ-butyro lactone                                                     9    130    1.02                                                                              --                                  Bicyclic Compounds                                                              ##STR130##      exo-2-bromo- norbornan                                                                    9    90     1.01                                                                              --                                  Furan and Pyran Derivatives                                                     ##STR131##      2-ethoxy-tetra hydrofuran                                                                 9    60     1.22                                                                              --                                   ##STR132##      3,4-dihydro-2- methoxy-2H-pyran                                                           9    60     1.06                                                                              --                                   ##STR133##      3,4-dihydro-2-ethoxy- 2H-pryan                                                            9    60     1.16                                                                              --                                   ##STR134##      2,5-dimethoxy-3- tetrahydrofuran carboxaldehyde                                           9    90     1.03 1.02 1.05 1.08                                                               --                                  __________________________________________________________________________

                                      TABLE IIb                                    __________________________________________________________________________     Beta-Cyclodextrin                                                              (DS by hydropropyl = average about 8,                                          ranged between 2-14)                                                                                               Column             Elution                                                     Length                                                                               Temperature  Order                   Structure          Compound         (m)   (°C.)                                                                           α                                                                             (if                     __________________________________________________________________________                                                            known)                  Alcohols                                                                                          2-amino-4-methyl 30            1.11 --                                         1-butanaol                                                   ##STR135##        trans-1,2-cyclo- hexanediol                                                                     20    120     1.04 --                       ##STR136##        trans-1,2-cyclo- heptanediol                                                                    20    120     1.03 --                      Nicotine Compounds                                                              ##STR137##                         20    120˜180                                                                          1.01 --                       ##STR138##                         20    140˜180                                                                          1.01 --                      Lactones                                                                        ##STR139##        pantoyl lactone  20    120     1.04 --                       ##STR140##        N-acetyl-homocystein thiolactone                                                                20    150     1.02 --                       ##STR141##        β-butyrolactone                                                                             9    70      1.03 --                       ##STR142##        α-methyl-γ-butyro lactone                                                            9    70      1.02 --                       ##STR143##        α-acetyl-α-methyl- γ-butyrolactone                                             9    90      1.06 --                      Furan and Pyran Derivatives                                                     ##STR144##        2-ethoxy-tetrahydro- furan                                                                       9    50      1.11 --                       ##STR145##        3,4-dihydro-2- methoxy-2H-pyran                                                                  9    50      1.03 --                       ##STR146##        3,4-dihydro-2-ethoxy- 2-H-pyran                                                                  9    50      1.05 --                       ##STR147##        tetrahydro-2-(2-propy- nyloxy)-2H-pyran                                                          9    60      1.05 --                       ##STR148##        2,5-dimethoxy-3- tetrahydrofuran carboxaldehyde                                                  9    100     1.05 1.10 1.09 1.02                                                                 --                      Epoxides and Glycidyl Analogues                                                 ##STR149##        1,2-epoxy-hexane 20    50      1.02 --                       ##STR150##        1,2-epoxyoctane  20    50      1.01 --                       ##STR151##        1,2-epoxy-7-octene                                                                              20    50      1.02 --                       ##STR152##        styrene oxide     9    80      1.06 --                       ##STR153##        limonene oxide   20    100     1.02 1.03                                                                           --                       ##STR154##        glycidyl methyl ether                                                                           20    45      1.04 --                       ##STR155##        glycidyl isopropyl ether                                                                        20    45      1.03 --                       ##STR156##        allyl glycidyl ether                                                                            20    45      1.05 --                       ##STR157##        t-butyl glycidyl ether                                                                          20    45      1.06 --                       ##STR158##        glycidyl methacrylate                                                                           20    70      1.01 --                      Bicyclic Compounds                                                              ##STR159##        2-acetyl-5-nornbornene                                                                           9    90      1.00 1.07                                                                           --                       ##STR160##        3-chloro-2-nor bornanone                                                                         9    90      1.00 1.05                                                                           --                       ##STR161##        exo-2-bromonor- bornanone                                                                        9    90      1.06 --                       Miscellaneous                                                                  ##STR162##        limonene         20    100     1.03 --                       ##STR163##        butyl lactate     9    60      1.02 --                       ##STR164##        solketal          9    80      1.04 --                       ##STR165##        α-(trichloromethyl)- benzyl acetate                                                       20    140     1.03 --                      __________________________________________________________________________

                                      TABLE IIc                                    __________________________________________________________________________     Gamma Cyclodextrin                                                             (DS by hydroxypropyl = average about 9, range between 2-16)                                           Column         Elution                                                         Length                                                                              Temperature                                                                              Order                                    Structure   Compound   (m)  (°C.)                                                                          α                                                                           (if known)                               __________________________________________________________________________      ##STR166## 2-(bromomethyl)-tetra hydrofuran                                                          9    70     1.02                                                                              --                                        ##STR167## limonene oxide                                                                            9    70     1.03 1.03                                                                         --                                        ##STR168## 3,4-dihydro-2-ethoxy 2H-pyran                                                             9    50     1.04                                                                              --                                       __________________________________________________________________________

EXAMPLE 5

This Example illustrates separating enantiomers using the media of dipentyl trifluoroacetyl ether derivative of cyclodextrin as made in Example 2 above.

Table III illustrates the separation results using the dipentyl trifluoroacetyl ether cyclodextrin (DP-TFA-CD) as the ether derivative of cyclodextrin.

The capacity factor of the first eluted enantiomer, k, is determined as follows: ##EQU2## where k'=retention time of the eluted solute; and k"=retention time of an unretained compound.

The column in the Table for "Stationary Phase" indicates which cyclodextrin was used for the ether derivative. "A" indicates that the ether derivative was dipentyl trifluoroacetyl alpha cyclodextrin; "B" that it was dipentyl trifluoroacetyl beta cyclodextrin; and "G" that it was dipentyl trifluoroacetyl ether gamma cyclodextrin.

The capillary tubes, the gas chromatographs and the determination of α were as set forth in Example 3 above.

                                      TABLE III                                    __________________________________________________________________________     Dipentyl Trifluoroacetyl Ether Cyclodextrin                                    (DPTFACD)                                                                                                                Stationary                           Structure             Compound   (°C.)                                                                      k  α                                                                           Phase                                __________________________________________________________________________     Alcohols, Diols and Polyols                                                     ##STR169##           2-butanol  40 40                                                                             0.69 0.82                                                                         1.22 1.16                                                                         B G                                   ##STR170##           3-butyn-2-ol                                                                              70 40                                                                             0.57 0.77                                                                         1.08 1.09                                                                         B G                                   ##STR171##           2-pentanol 40 40                                                                             1.31 1.61                                                                         1.26 1.27                                                                         B G                                   ##STR172##           2-hexanol  40 3.75                                                                              1.31                                                                              G                                     ##STR173##           4-methyl-2-pentanol                                                                       40 40                                                                             1.58 2.18                                                                         1.19 1.17                                                                         B G                                   ##STR174##           2-heptanol 40 40                                                                             7.85 8.39                                                                         1.25 1.26                                                                         B G                                   ##STR175##           3-heptanol 40 4.14                                                                              1.08                                                                              G                                     ##STR176##           2-octanol  70 40                                                                              2.80 20.7                                                                        1.06 1.15                                                                         B G                                   ##STR177##           2-decanol  70 40                                                                             13.4 67.1                                                                         1.18 1.22                                                                         B G                                   ##STR178##           1,2-propanediol                                                                           70 70                                                                             2.06 1.67                                                                         1.08 1.18                                                                         A B                                   ##STR179##           1,3-butanediol                                                                            70 70 70                                                                          4.92 4.86 4.79                                                                    1.06 1.14 1.21                                                                    A B G                                 ##STR180##           (2R,3R)- and (2S,3S)-butanediol                                                           70 1.43                                                                              1.58                                                                              G                                     ##STR181##           1,2-pentanediol                                                                           70 70                                                                             7.77 3.54                                                                         1.09 1.03                                                                         A B                                   ##STR182##           1,4-pentanediol                                                                           90 70                                                                              5.23  13.6                                                                       1.06 1.05                                                                         B G                                   ##STR183##           1,2-hexanediol                                                                            70 22.5                                                                              1.08                                                                              A                                     ##STR184##           1,2-octanediol                                                                            90 10.6                                                                              1.05                                                                              B                                     ##STR185##           trans-1,2-cyclo- hexanediol                                                               70 12.0                                                                              1.58                                                                              G                                     ##STR186##           trans-1,2-cyclo- heptanediol                                                              70 19.6                                                                              1.15                                                                              G                                     ##STR187##           trans-1,2-cyclo- ocanediol                                                                70 36.0                                                                              1.12                                                                              G                                     ##STR188##           1,2,4-butanetriol                                                                         110                                                                               8.10                                                                              1.02                                                                              B                                     ##STR189##           1,2,6-hexanetriol                                                                         110                                                                               23.3                                                                              1.07                                                                              B                                     ##STR190##           arabitol   100                                                                               20.6                                                                              1.06                                                                              B                                    Amino Alcohols                                                                  ##STR191##           2-amino-1-propanol                                                                        100 110 100                                                                       5.88  3.81 6.53                                                                   1.07 1.16 1.99                                                                    A B G                                 ##STR192##           1-amino-2-propanol                                                                        100 110 100                                                                       6.25 4.76 5.71                                                                    1.14 1.03 1.20                                                                    A B G                                 ##STR193##           2-amino-1-butanol                                                                         110 110 100                                                                       7.31 3.42 7.04                                                                    1.08 1.06 1.17                                                                    A B G                                 ##STR194##           2-amino-1-pentanol                                                                        110 100                                                                           4.33 8.93                                                                         1.14 1.14                                                                         B G                                   ##STR195##           2-amino-3-methyl- 1-butanol                                                               110 100                                                                           2.95 6.43                                                                          1.07 1.19                                                                        B G                                   ##STR196##           2-amino-1-hexanol                                                                         110 110 100                                                                       15.0  7.57 10.4                                                                   1.04 1.11 1.1                                                                     A B G                                 ##STR197##           leucinol   120 110                                                                           3.81 4.33                                                                         1.06 1.14                                                                         A B                                   ##STR198##           3-amino-1,2- propanediol                                                                  140                                                                               6.14                                                                              1.12                                                                              B                                    Amines                                                                          ##STR199##           2-aminobutane                                                                             80 4.29                                                                              1.04                                                                              G                                     ##STR200##           2-aminopentane                                                                            80 3.75                                                                              1.03                                                                              A                                     ##STR201##           1,3-dimethylbutyl- amine                                                                  80 4.88                                                                              1.06                                                                              A                                     ##STR202##           2-aminoheptane                                                                            90 80                                                                             10.3 19.6                                                                         1.07 1.02                                                                         A G                                   ##STR203##           3-aminoheptane                                                                            90 80                                                                              7.19 13.4                                                                        1.06 1.03                                                                         A G                                   ##STR204##           1,5-dimethylhexyl- amine                                                                  90 80                                                                             10.1 28.4                                                                         1.01 1.01                                                                         A G                                   ##STR205##           1-cyclohexylethyl- amine                                                                  100                                                                               11.2                                                                              1.08                                                                              B                                     ##STR206##           1-phenylethyl- amine                                                                      100                                                                               12.9                                                                              1.05                                                                              B                                     ##STR207##           1-aminoindan                                                                              140                                                                               4.06                                                                              1.06                                                                              A                                     ##STR208##           1,2,3,4-tetrahydro- 1-naphthylamine                                                       140 140                                                                           6.12 8.21                                                                         1.04 1.03                                                                         A G                                  Carboxylic Acid Esters                                                          ##STR209##           lactic acid methyl ester                                                                  50 6.79                                                                              1.47                                                                              G                                     ##STR210##           lactic acid butyl ester                                                                   60 15.0                                                                              1.05                                                                              G                                     ##STR211##           mandelic acid methyl ester                                                                110                                                                               4.00                                                                              1.04                                                                              B                                     ##STR212##           mandelic acid ethyl ester                                                                 110 120                                                                           3.57 4.11                                                                         1.03 1.09                                                                         B G                                   ##STR213##           D,L-tartaric acid dimethyl ester                                                          90 2.79                                                                              1.04                                                                              B                                     ##STR214##           D,L-tartaric acid diisopropyl ester                                                       90 8.57                                                                              1.07                                                                              B                                     ##STR215##           2-chloropropionic acid methyl ester                                                       60 60                                                                             6.25 5.00                                                                         2.69 2.14                                                                         B G                                   ##STR216##           2-bromobutyric acid sec-butyl ester.sup.+                                                 80 10.0 10.6                                                                         1.22 1.07                                                                         G                                     ##STR217##            2-bromopropionic acid methyl ester                                                       80 80                                                                             9.38 2.44                                                                         1.12 1.47                                                                         B G                                   ##STR218##           2-bromopropionic acid ethyl ester                                                         80 80                                                                             3.85 3.50                                                                         1.14 1.16                                                                         B G                                   ##STR219##           2-bromopropionic acid butyl ester                                                         80 8.00                                                                              1.05                                                                              G                                     ##STR220##           2-bromopropionic acid pentyl ester                                                        80 15.6                                                                              1.04                                                                              G                                     ##STR221##           2-bromopropionic acid hexyl ester                                                         80 30.9                                                                              1.04                                                                              G                                     ##STR222##           2-bromopropionic acid sec-butyl ester.sup.+                                               60 80                                                                             12.0 12.5  4.89  5.39                                                             1.25 1.05 1.29 1.02                                                               B G                                   ##STR223##           2-bromopropionic acid sec-pentyl ester.sup.+                                              60 80                                                                             21.5 22.9  8.00  8.37                                                             1.28 1.12 1.24 1.05                                                               B G                                   ##STR224##           2-bromopropionic acid sec-hexyl ester.sup.+                                               60 80                                                                             45.6 49.5 14.7 15.1                                                               1.26 1.10 1.25 1.07                                                               B G                                   ##STR225##           2-bromopropionic acid sec-heptyl ester.sup.+                                              90 15.1 15.4                                                                         1.16 1.04                                                                         G                                     ##STR226##           2-bromopropionic acid sec-octyl ester.sup.+                                               90 29.3 29.6                                                                         1.16 1.03                                                                         G                                    Halohydrocarbons                                                                ##STR227##           2-bromo-1-chloro- propane                                                                 40 40 30                                                                          4.31 4.00 7.64                                                                    1.06 1.12 1.05                                                                    A B G                                 ##STR228##           2-chlorobutane                                                                            30 30                                                                             0.73 0.91                                                                         1.10 1.12                                                                         A G                                   ##STR229##           3-chloro-1-butene                                                                         30 30                                                                             0.72 0.82                                                                         1.06 1.13                                                                         A G                                   ##STR230##           2-bromobutane                                                                             30 35 30                                                                          1.65 1.74 2.44                                                                    1.38 1.04 1.09                                                                    A B G                                 ##STR231##           2-iodobutane                                                                              40 60 30                                                                          2.92 1.31 6.36                                                                    1.04 1.24 1.06                                                                    A B G                                 ##STR232##           1,2-dichlorobutane                                                                        60 60                                                                             2.45 0.89                                                                         1.09 1.04                                                                         B G                                   ##STR233##           (2R,3R) and (2S,3S) 2,3-dichlorobutane                                                    60 60                                                                             2.15 2.05                                                                         1.59 1.60                                                                         B G                                   ##STR234##           1,2-dibromobutane                                                                         90 70 60                                                                          4.13 4.54 8.57                                                                    1.03 1.03 1.13                                                                    A B G                                 ##STR235##           1,3-dibromobutane                                                                         90 70 60                                                                           6.38  7.38 13.6                                                                  1.02 1.05 1.79                                                                    A B G                                 ##STR236##           2-bromopentane                                                                            35 30                                                                             3.44 8.27                                                                         1.03 1.32                                                                         B G                                   ##STR237##           2-bromoheptane                                                                            70 50                                                                             4.15 8.57                                                                         1.07 1.18                                                                         B G                                   ##STR238##           2-bromo-1-phenyl- propane                                                                 100                                                                               6.39                                                                              1.06                                                                              G                                    Epoxides, Glycidyl Analogoues                                                  and Haloepihydrins                                                              ##STR239##           1,2-epoxyhexane                                                                           50 40                                                                             3.31 5.36                                                                         1.02 1.10                                                                         A G                                   ##STR240##           1,2-epoxyoctane                                                                           50 22.3                                                                              1.04                                                                              A                                     ##STR241##           1,2-epoxydecane                                                                           90 9.88                                                                              1.02                                                                              A                                     ##STR242##           1,2-epoxydodecane                                                                         90 108                                                                               1.02                                                                              A                                     ##STR243##           1,2-epoxytetradecane                                                                      100                                                                               125                                                                               1.02                                                                              A                                     ##STR244##           styrene oxide                                                                             80 80                                                                              5.57 10.7                                                                        1.01 1.57                                                                         B G                                   ##STR245##           limonene oxide.sup.+                                                                      80 8.57 8.57                                                                         1.06 1.10                                                                         G                                     ##STR246##           trans-stilbene oxide                                                                      140                                                                               20.0                                                                              1.02                                                                              G                                     ##STR247##           ±-1,3-butadiene diepoxide                                                              80 3.93                                                                              1.11                                                                              G                                     ##STR248##           glycidol   60 4.29                                                                              1.06                                                                              G                                     ##STR249##           glycidyl methyl ether                                                                     40 45                                                                             3.33 5.89                                                                         1.04 1.16                                                                         B G                                   ##STR250##           glycidyl isopropyl ether                                                                  40 45                                                                              6.00 10.5                                                                        1.04 1.04                                                                         B G                                   ##STR251##           allyl glycidyl ether                                                                      50 45                                                                              3.23 21.6                                                                        1.02 1.42                                                                         A G                                   ##STR252##           n-butyl glycidyl ether                                                                    50 45                                                                             11.8 23.2                                                                         1.02 1.04                                                                         B G                                   ##STR253##           t-butyl glycidyl ether                                                                    45 14.5                                                                              1.06                                                                              G                                     ##STR254##           glycidyl acrylate                                                                         80 10.1                                                                              1.14                                                                              G                                     ##STR255##           glycidyl methacrylate                                                                     80 10.4                                                                              1.04                                                                              G                                     ##STR256##           (2S,3S) and (2R,3R)-2- methyl-3-phenyl- glycidol                                          100                                                                               13.6                                                                              1.06                                                                              G                                     ##STR257##           epifluorohydrin                                                                           30 2.50                                                                              1.02                                                                              G                                     ##STR258##           epichlorohydrin                                                                           60 4.82                                                                              1.20                                                                              G                                     ##STR259##           epibromohydrin                                                                            60 4.82                                                                              1.20                                                                              G                                    Lactones                                                                        ##STR260##           β-butyrolactone                                                                      100 70 80                                                                          2.73 13.7  7.14                                                                  1.14 1.62 1.20                                                                    A B G                                 ##STR261##           3-hydroxy-4,4,4- trichlorobutyric-β- lactone                                         120 100                                                                            3.50 15.6                                                                        1.11 1.19                                                                         B G                                   ##STR262##           α-methyl-γ-butyro- lactone                                                    110 110                                                                            3.03 11.5                                                                        1.29 1.07                                                                         A B                                   ##STR263##           α-acetyl-α-methyl-γ- butyrolactone                           7          120                                                                               6.28                                                                              1.59                                                                              B                                     ##STR264##           pantoyl lactone                                                                           120 120                                                                            4.25 1.57                                                                        1.04 1.05                                                                         A B                                   ##STR265##           β,β'-dimethyl-γ- (hydroxymethyl)-                              γ-butyrolactone                                                                     120                                                                               12.3                                                                              1.10                                                                              B                                     ##STR266##           γ-nonanoiclactone                                                                   160                                                                               4.07                                                                              1.06                                                                              B                                     ##STR267##           α-bromo-γ-valero- lactone.sup.+                                               150                                                                               4.64 5.36                                                                         1.09 1.09                                                                         G                                     ##STR268##           γ-decanolactone                                                                     140                                                                               9.24                                                                              1.05                                                                              G                                     ##STR269##           δ-decanolactone                                                                     140                                                                               11.8                                                                              1.02                                                                              G                                    Furan and Pyran Derivatives                                                     ##STR270##           2-ethoxy-tetra- hydrofuran                                                                40 40 45                                                                          3.63 3.08 3.04                                                                    1.03 1.05 1.20                                                                    A B G                                 ##STR271##           3,4-dihydro-2- methyloxy-2H-pyran                                                         40 40 45                                                                          3.38 4.33 4.29                                                                    1.07 1.22 1.13                                                                    A B G                                 ##STR272##           3,4-dihydro-2- ethoxy-2H-pyran                                                            40 40 45                                                                          5.85 5.92 6.07                                                                    1.04 1.06 1.09                                                                    A B G                                 ##STR273##           tetrahydro-2- (2-propynyloxy)- 2H-pyran                                                   80 70                                                                             3.71 8.14                                                                         1.01 1.08                                                                         B G                                   ##STR274##           2-(chloromethyl)- tetrahydropyran                                                         80 70                                                                             3.24 8.04                                                                          1.28 1.03                                                                        B G                                   ##STR275##           2-(bromomethyl)- tetrahydropyran                                                          80 6.07                                                                              1.25                                                                              B                                     ##STR276##           3-hydroxy-tetra- hydropyran                                                               50 7.14                                                                              1.18                                                                              G                                     ##STR277##           trans-2,5-dimethyl- tetrahydrofuran                                                       45 8.57                                                                              1.06                                                                              G                                     ##STR278##           trans-2,5-dimethoxy- tetrahydrofuran                                                      60 1.46                                                                              1.92                                                                              G                                    Nicotine Compounds                                                              ##STR279##           nicotine   160                                                                               2.14                                                                              1.13                                                                              B                                     ##STR280##           1-methyl-2-phenyl- pyrrolidine                                                            160                                                                               1.43                                                                              1.20                                                                              B                                     ##STR281##           2-benzylpyrrolidine                                                                       140                                                                               10.2                                                                              1.06                                                                              B                                    Bicyclic Compounds                                                              ##STR282##           endo- and exo-2- acetyl-5-norbor- nene.sup.+                                              110 100                                                                           1.73 2.45 4.39 6.32                                                               1.03 1.00 1.11 1.09                                                               B G                                   ##STR283##           endo- and exo-2- aminonorbornane.sup.+                                                    110                                                                               4.71 6.21                                                                         1.18 1.10                                                                         B                                     ##STR284##           endo- and exo-2- benzoyl-5-norbor- nene.sup.+                                             120                                                                               34.6  41.8                                                                        1.00 1.04                                                                         G                                     ##STR285##           exo-2-bromo- norbornane                                                                   70 11.3                                                                              1.02                                                                              G                                     ##STR286##           exo-2-chloro- norbornane                                                                  70 5.79                                                                              1.01                                                                              G                                     ##STR287##           endo- and exo-3- chloro-2- norbornanone.sup.+                                             120                                                                                6.57 10.2                                                                        1.06 1.04                                                                         G                                     ##STR288##           DL-isoborneol                                                                             70 8.68                                                                              1.05                                                                              G                                    Ketones                                                                         ##STR289##           2-methyl-cyclo- hexanone                                                                  80 5.29                                                                              1.08                                                                              G                                     ##STR290##           carvone    90 110 100                                                                        13.6  7.05 11.4                                                                   1.04 1.09 1.01                                                                    A B G                                Miscellaneous                                                                   ##STR291##           3-chloro-2-butanone                                                                       50 60                                                                             3.46 2.43                                                                         1.62 1.59                                                                         B G                                   ##STR292##           solketal   90 60                                                                             1.57 7.86                                                                         1.04 1.07                                                                         B G                                   ##STR293##           3-chloro-1,2- propanediol                                                                 90 3.52                                                                              1.09                                                                              B                                     ##STR294##           2-chloropropionitrile                                                                     80 70                                                                             1.93 1.65                                                                         1.07 1.06                                                                         B G                                   ##STR295##           2-bromopropionitrile                                                                      80 70                                                                             4.07 4.13                                                                         1.14 1.06                                                                         B G                                   ##STR296##           1-chloro-2-propanol                                                                       70 5.21                                                                              1.02                                                                              G                                     ##STR297##           4-phenyl-1,3-dioxane                                                                      120                                                                               7.36                                                                              1.04                                                                              G                                     ##STR298##           2-chlorocyclo- pentanone                                                                  90 110                                                                            12.6  2.47                                                                        2.51 1.33                                                                         B G                                   ##STR299##           2-amino-1- methoxy-propane                                                                90 100                                                                            1.90 1.71                                                                         1.25 1.08                                                                         B G                                   ##STR300##           2-chlorocyclo- hexanone                                                                   90 110                                                                            21.2  4.26                                                                        1.06 1.10                                                                         B G                                   ##STR301##           2-methyl-piperidin                                                                        100                                                                               5.54                                                                              1.13                                                                              G                                     ##STR302##           3-methyl-piperidin                                                                        100                                                                               5.00                                                                              1.14                                                                              G                                     ##STR303##           mandelonitrile                                                                            100                                                                               5.21                                                                              1.10                                                                              G                                     ##STR304##           ethyl-3-phenyl- glycidate.sup.+                                                           120                                                                               16.6 23.8                                                                          1.05 1.04                                                                        G                                    __________________________________________________________________________      .sup.+ compound containing more than one chiral center.                  

EXAMPLE 6

This Example illustrates retention and selectivity results of homologous racemic amines, diols, and α-halocarboxylic acid esters using the media of dipentyl trifluoroacetyl ether cyclodextrin as made in Example 2 above. These results are set forth in the following Table IV.

The capillary tubes, the gas chromatographs and the determination of α were as set forth in Example 3 above.

The capacity factor and the indication of the base cyclodextrin used were as set forth in Example 4 above.

                                      TABLE IV                                     __________________________________________________________________________                                            Stationary                              Structure           Compound  (°C.)                                                                      k  α                                                                           Phase                                   __________________________________________________________________________      ##STR305##         2-aminobutane                                                                            90  1.55                                                                             1.14                                                                              B                                        ##STR306##         2-amino-3,3- dimethylbutane                                                              90  2.10                                                                             1.22                                                                              B                                        ##STR307##         2-aminopentane                                                                           90  2.40                                                                             1.22                                                                              B                                        ##STR308##         1,3-dimethylbutyl- amine                                                                 90  2.80                                                                             1.22                                                                              B                                        ##STR309##         2-aminoheptane                                                                           90  8.15                                                                             1.22                                                                              B                                        ##STR310##         1,5-dimethylhexyl- amine                                                                 90 12.5                                                                              1.22                                                                              B                                        ##STR311##         1,2-propanediol                                                                          70  1.61                                                                             1.49                                                                              G                                        ##STR312##         1,2-pentanediol                                                                          70  3.50                                                                             1.23                                                                              G                                        ##STR313##         1,2-hexanediol                                                                           70  6.64                                                                             1.23                                                                              G                                        ##STR314##         1,2-octanediol                                                                           70 29.3                                                                              1.23                                                                              G                                        ##STR315##         2-bromobutyric acid methyl ester                                                         80 80                                                                              5.07 6.71                                                                        1.56 1.57                                                                         B G                                      ##STR316##         2-bromobutyric acid ethyl ester                                                          80 80                                                                              5.25 9.93                                                                        1.29 1.16                                                                         B G                                      ##STR317##         2-bromobutyric isopropyl ester                                                           80  6.57                                                                             1.08                                                                              G                                        ##STR318##         2-bromobutyric acid butyl acid                                                           80 80                                                                             15.5 20.5                                                                         1.16 1.09                                                                         B G                                      ##STR319##         2-bromobutyric acid pentyl ester                                                         80 80                                                                             32.3 39.5                                                                         1.16 1.08                                                                          B G                                     ##STR320##         2-bromobutyric acid hexyl ester                                                          80 80                                                                             66.4 80.0                                                                         1.16 1.09                                                                         B G                                     __________________________________________________________________________

EXAMPLE 7

A test was made to determine if the stereochemistry of the ether side chain has any effect on separation.

Three different media were prepared, permethyl-(S)-hydroxypropyl-beta-cyclodextrin; permethyl-(R)-hydroxypropyl-beta cyclodextrin; and permethyl-(racemic)-hydroxypropyl-beta-cyclodextrin.

Three identical columns were coated with these materials and a series of tests were run. Each of the three columns produced essentially identical separations.

It will be understood that it is intended to cover all changes and modifications of the preferred embodiments herein chosen for the purpose of illustration which do not constitute a departure from the spirit and scope of the invention. 

What is claimed is:
 1. A composition for separation of optical isomers in a gas chromatographic column, said composition being a dialkyl trifluoro ester ether of cyclodextrin having about 20% to about 80% of the hydrogens of the hydroxyl groups of the cyclodextrin substituted with alkyl side chains and at least about 90% of the hydrogens of the remaining hydroxyl groups of the alkylated ether of cyclodextrin substituted with a fluorinated ester group.
 2. The composition of claim 1 wherein the fluorinated ester group is selected for the group consisting of trifluorinated acetyl, trifluorinated butyryl, and trifluorinated propanoic.
 3. The composition of claim 2 wherein the alkyl group is pentane.
 4. The composition of claim 2 wherein the cyclodextrin is gamma-cyclodextrin.
 5. The composition of claim 2 wherein the cyclodextrin is beta-cyclodextrin.
 6. The composition of claim 2 wherein the cyclodextrin is alpha-cyclodextrin.
 7. A composition made by the process comprising:a) etherifying a cyclodextrin with an alkyl halide to form an ether of cyclodextrin having between 20% and about 80% of the hydrogens of the hydroxyl groups of the cyclodextrin substituted by the alkyl of the alkyl halide; and b) esterifying at least about 90% of the hydrogens of the remaining hydroxyl groups of the ether derivative of cyclodextrin with a fluorinated anhydride.
 8. The composition of claim 7 wherein the cyclodextrin is selected from the group consisting of alpha-cyclodextrin, beta-cyclodextrin, and gamma-cyclodextrin.
 9. The composition of claim 7 wherein the alkyl halide is selected form the group consisting of propyl bromide, propyl chloride, butyl chloride, butyl bromide, pentyl bromide and pentyl chloride.
 10. The composition of claim 7 wherein the fluorinated anhydride is selected from the group consisting of trifluoroacetic anhydride, trifluorobutanoic anhydride, and trifluoropropanoic anhydride.
 11. A composition for separation of optical isomers in a gas chromatographic column, said composition being a dialkyl trifluoro ester ether of cyclodextrin.
 12. A composition of claim 11 wherein the cyclodextrin is selected from the group consisting of alpha-, beta- and gamma-cyclodextrin.
 13. The composition of claim 11 wherein the alkyl group is selected from the group consisting of methyl, ethyl, propyl, butyl, pentyl and hexyl.
 14. The composition of claim 11 wherein the fluorinated ester group is selected from trifluorinated acetyl, trifluorinated butyl and trifluorinated propanoic. 